Yes, you can. Systemwide can be used to route your system audio (like your music players or internet browser) through the calibration and it can also be used the same way with your DAW. Just make sure to set Sonarworks Systemwide (Sonarworks Virtual Audio Device on Windows) as your output (but not the input) device in your DAW settings.
There are certain advantages to using your DAW with Systemwide instead of the Reference Plug-in. For one, with Systemwide you don't have to remember to bypass your calibration before rendering your mix. On the other hand, adding Systemwide in the chain between your DAW and audio interface results in increased latency (around 20-50ms) which can become an issue when recording audio or MIDI.
So if you want to use the calibration while tracking music, we suggest using the Reference Plug-in. For mixing or if the latency added latency is not an issue, we suggest using Systemwide.
Please note that Systemwide will not work with Cubase on Windows because Systemwide currently supports Windows native drivers only while Cubase works exclusively with ASIO drivers. We plan to add ASIO driver support on Systemwide in the future. Right now a similar functionality can be achieved using the Reference plugin in the Cubase Control room. Read more about it here.